Severe burns leave patients vulnerable to infections since their body can be exposed to microorganisms through open wounds. Our lab has previously shown that fms-like tyrosine kinase-3 ligand (Flt3L) treatment after severe burn injury enhances bacterial clearance resulting in increased resistance to a burn wound infection. In vivo studies show that Flt3L treatment enhances neutrophil responses to burn wound infection; in vitro studies show that Flt3L enhances neutrophil migration in a DC-dependent manner. Neutrophils and dendritic cells (DCs) both play important roles in fighting infections in burn wounds and can interact and provide bidirectional activation. In order to determine if neutrophil-DC interactions and functions are altered by Flt3L, in vitro studies would be useful. MPRO cells are a neutrophil-like cell line that could provide an abundance source of cells for neutrophil studies. The purpose of this project was to characterize MPRO cells for various neutrophil characteristics. The expression of neutrophil proteins and in vitro migration, in the presence and absence of DCs, was examined. While there were some differences between neutrophils and MPRO cells, we found that MRPO cells may be suitable for studying migration and interactions with DCs in vitro.