Greens Bayou plays a significant role in the environmental balance and survival of both native flora and fauna in Harris County. However, highway runoff water that flows into the bayou and the construction of concrete retaining walls change the chemistry and fluid dynamics of the water system. This study will determine the concentration of ubiquitous microorganism, E. Coli, and a chemical contaminant, polycyclic aromatic hydrocarbon (PAH) in various part of the bayou. E. Coli is to be measured cultivating the water and seeing the amount of concentration of E. Coli from the sample. The detection of PAH will be used by a gas chromatography-mass spectrometry. The daphnia will be used as a habitat indicator to test the water. The heart rate of the daphnia will be measure to a control daphnia in spring water and see the heart rate to determine if it is suitable for life. Environmental quality is important in the sustainable growth of Harris County. Detailed study of the bayou will help the city planners understand the relationship between growth and sustainability.